Figure 7

Relative expression of candidate genes in Phenacoccus solenopsis 72 h post injection of respective 10 µg of dsRNA in comparison to GFP control (A) calcitonin 50.9% kd (knockdown), (B) aquaporin (24.4% kd), (C) VATpase (61.99% kd), (D) IAP (Inhibitor of Apoptosis) (65.58% kd) (E) bursicon(85.7% kd), (F) SNF7 (40.8% kd), (G) chitin synthase (78.02% kd), (H) α-amylase (83.6% kd). The expression level of each gene has been normalized with 28 s as internal control. The error bars represent the standard deviation (3 replicates) and *represents significant differences in mRNA transcripts compared to GFP control (P ≤ 0.05, Student’s t-test).