Figure 5

Biological prediction website and dual-luciferase reporter gene assay verify miR-495 targets and negatively regulates AQP1. (A) predicted binding site of miR-495 in AQP1-3′UTR; (B) luciferase activity of cells transfected with AQP1-3′UTR-Wt and AQP1-3′UTR-Mut; *p < 0.05 vs. the control group; miR-495, microRNA-495; AQP1, aquaporin-1; UTR, untranslated region; Wt, wild type; Mut, mutant type.