Table 2 Primer sequences of related genes for RT-qPCR.

From: MicroRNA-495 downregulates AQP1 and facilitates proliferation and differentiation of osteoblasts in mice with tibial fracture through activation of p38 MAPK signaling pathway

Gene

Sequence

mmu-miR-495

Forward: 5′-AAACAAACATGGTGCA-3′

Reverse: 5′-GAGCAGGCTGGAGAA-3′

AQP1

Forward: 5′-AGGCTTCAATTACCCACTGGA-3′

Reverse: 5′-CTTTGGGCCAGAGTAGCGAT-3′

p38 MAPK

Forward: 5′-TGACCCTTATGACCAGTCCTTT-3′

Reverse: 5′-GTCAGGCTCTTCCACTCATCTAT-3′

Cyclin D1

Forward: 5′-GCGTACCCTGACACCAATCTC-3′

Reverse: 5′-ACTTGAAGTAAGATACGGAGGGC-3′

PCNA

Forward: 5′-TTGCACGTATATGCCGAGACC-3′

Reverse: 5′-GGTGAACAGGCTCATTCATCTCT-3′

OCN

Forward: 5′-GGCTTAAAGACCGCCTACAG-3′

Reverse: 5′-GAGAGGACAGGGAGGATCAA-3′

OPN

Forward: 5′-ATCTCACCATTCGGATGAGTCT-3′

Reverse: 5′-TGTAGGGACGATTGGAGTGAAA-3′

RUNX2

Forward: 5′-GACTGTGGTTACCGTCATGGC-3′

Reverse: 5′-ACTTGGTTTTTCATAACAGCGGA-3′

GAPDH

Forward: 5′-AGGTCGGTGTGAACGGATTTG-3′

Reverse: 5′-GGGGTCGTTGATGGCAACA-3′

U6

Forward: 5′-CTCGCTTCGGCAGCACA-3′

Reverse: 5′-AACGCTTCACGAATTTGCGT-3′

  1. Note: RT-qPCR, reverse transcription quantitative polymerase chain reaction; miR-495, microRNA-495; p38 MAPK; p38 mitogen-activated protein kinase; PCNA, proliferating cell nuclear antigen; OCN, osteocalcin; OPN, osteopontin; RUNX2, runt-related transcription factor 2; GAPDH, glyceraldehyde-3-phosphate dehydrogenase.