Figure 3 | Scientific Reports

Figure 3

From: Biochemical and biophysical characterization of purified native CD20 alone and in complex with rituximab and obinutuzumab

Figure 3

Behavior in solution of purified CD20. (A) Gel filtration chromatography (Superose 10/300 Increase) on purified CD20. Chromatogram is shown in Top. Protein fractions were analyzed by SDS-PAGE and western blot (Bottom). Vo indicates the void volume of the column. * and **indicates CD20 and detergent elution volume, respectively. CALX-173-GK detergent absorbs at 280 nm. Full length SEC chromatogram is shown in Fig. S4. (B) Distribution of sedimentation coefficient c(s) for the CALX-173-GK micelles at 0.5% (w/v) detergent. Interference and absorbance detection (250 nm) are shown. (C) Normalized distribution of sedimentation coefficient c(s) for CD20 protein (0.044 and 0.5 mg/ml) and detergent micelles (0.5% CALX-173-GK and 0.05% (w/v) DDM) with absorbance (250 nm) and interference detection. Full-length gels and blots are included in a Supplementary Information file (Fig. S2).

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