Figure 1

Morphological changes in melanocytes treated with PAM. The melanocytes were incubated for (A) 24 and (B) 48 h at a PAM dose of 20 min. The live cell number was counted to evaluate the effects of PAM on proliferation after (C) 24 and (D) 48 h of incubation. To confirm the cytotoxicity of PAM, (E) WST-1 and (F) LDH release assays were performed after incubation for 24 and 48 h.