Figure 2 | Scientific Reports

Figure 2

From: Mass spectrometric identification of candidate RNA-binding proteins associated with Transition Nuclear Protein mRNA in the mouse testis

Figure 2

Tnp mRNA-associated proteins are expressed early in mouse testis development. (a) Diagram of germ cell composition of the mouse testis at different developmental stages. The only germ cells in a testis from a mouse that is 6 dpp are spermatogonia. At 10 dpp, spermatogonia and early spermatocytes in meiosis (preleptotene, leptotene, and zygotene spermatocytes) are present. As development proceeds, pachytene spermatocytes (14 dpp), diplotene spermatocytes (18 dpp), round spermatids (22 dpp), elongating spermatids (30 dpp) and fully differentiated spermatozoa (>90 dpp adult) are included in the testicular repertoire. (b) Candidate Tnp regulatory proteins are expressed early in spermatogenesis. Relevant bands from representative western blots of mouse testis extracts probed with antibodies recognizing TNP1, TNP2 and the six candidate proteins (CSDE1, DAZL, ELAVL1, IGF2BP3, LRPPRC and MATR3) are shown. Extracts were prepared from mouse testes at seven distinct developmental time points (6, 10, 14, 20, 25, 30 and >90 dpp). Each experiment was performed on three sets of biological replicates. A blot probed for the housekeeping protein GAPDH is shown as a loading reference. Antibodies for TNP1, TNP2, and the six candidate proteins were specific for the respective target proteins by western blot. Full-length blots are presented in Supplementary Fig. S3.

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