Figure 3 | Scientific Reports

Figure 3

From: Mass spectrometric identification of candidate RNA-binding proteins associated with Transition Nuclear Protein mRNA in the mouse testis

Figure 3

TNP1, TNP2 and candidate RNA-binding proteins are expressed in differentiating germ cells. (a) TNP protein expression is restricted to spermatids in specific stages of the mouse seminiferous epithelium. Drawings (left) illustrate the cell types present at stages I, VII–VIII and XII of the spermatogenic cycle (images courtesy of NIEHS). Representative tubule sections from adult mouse testis are shown stained with lectin (magenta) to identify acrosomes for staging of spermatids and DAPI (blue) to identify nuclei. Immunofluorescent detection of TNP1 (red) and TNP2 (green) are shown as individual panels and as a merged image with DAPI staining. (b) Tnp mRNA-associated proteins are expressed by adult mouse germ cells. Representative adult mouse testis tubule sections with immunofluorescent detection of candidate proteins: CSDE1, DAZL, ELAVL1, IGF2BP3, LRPPRC and MATRIN3. A representative negative control, where primary antibody was omitted from the staining protocol, is also shown. Immunofluorescent detection of candidate proteins (green) and DAPI staining (blue) are shown as individual panels and as a merged image. Zoomed views of the yellow-boxed areas are also shown to the right of the merged images.

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