Figure 3 | Scientific Reports

Figure 3

From: Novel breast cancer screening: combined expression of miR-21 and MMP-1 in urinary exosomes detects 95% of breast cancer without metastasis

Figure 3

Western blot analysis of MMP-1 and CD63 in urinary exosomes in patients with BC and healthy controls. (A) BC patients (n = 9); No. 1–9, and (B) Healthy control (n = 9); No. 1–9 (shown in Table 1). One-sixth of the urinary exosome extracted from 2 mL urine was applied to the wells. Anti-MMP-1 antibody (1:1000) and anti-CD63 antibody (1:1000) were used. Samples derived from the same experiment with blots processed in parallel. Strict loading control was performed on all blots. Full-length blots are presented in Supplementary Fig. 1. BC, breast cancer.

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