Figure 3 | Scientific Reports

Figure 3

From: Non-cytotoxic hydroxyl-functionalized exfoliated boron nitride nanoflakes impair the immunological function of insect haemocytes in vivo

Figure 3

The in vivo short- and long-term haemocyte bioassay showing viable, adhesive haemocytes isolated from the insects injected with h-BN-OH-n or topically exposed to h-BN-OH-n. Haemocytes: 2 hours after saline injection (A) and 2 days (D) after topical application of saline (controls), 2 hours after injection of 2 ng (B) or 2 µg (C) of h-BN-OH-n, 2 days after topical application of 2 ng (E) and 2 µg (F) of h-BN-OH-n. The active caspases (1–9) were stained with SR-VAD-FMK (no red  =  no active caspases), the F-actin cytoskeleton was stained with Oregon Green 488 phalloidin (green) and DNA was stained with DAPI (blue). Scale bars: 20 µm.

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