Figure 7 | Scientific Reports

Figure 7

From: Prokaryotic soluble overexpression and purification of oncostatin M using a fusion approach and genetically engineered E. coli strains

Figure 7

Inhibitory effect of the commercial and purified OSM proteins on the differentiation of CD4+ T cells into Th17 cells. CD4+ T cells were treated with commercial or purified OSMs under Th17 cell-polarising conditions (n = 3). After 72 h, the culture supernatants were analysed by ELISA to determine the levels of secreted IL-17 (A), and the cells treated with 10 ng/mL OSM proteins were analysed by flow cytometry to determine the intracellular levels of IL-17 in differentiated CD4+ T cells (B). (+), commercial OSM; His, OSM purified from His-OSM; MBP, OSM purified from MBP-OSM.

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