Figure 3
From: Male mice, caged in the International Space Station for 35 days, sire healthy offspring
Characterization of fertilized eggs and F1 mice from AG and MG males. (a) Embryogenesis of fertilized eggs. Embryogenesis was observed for 4 days after IVF. (b) Immunostaining of OCT4 and CDX2 in blastocysts. OCT4 and CDX2 were used as markers for ICM and TE cells in blastocysts, respectively. Scale bars are 100 µm. (c) Quantification of OCT4- and CDX2-positive cells in blastocysts. Data are the mean ± standard deviation. N.S.: not significant. (d) Rates of delivered pups. Fertilized eggs obtained by IVF were transferred into pseudopregnent females. (e) Offspring from fertilized eggs with MG spermatozoa. (f) Growth curve of F1 mice. Body weight was monitored by postnatal day 63. “M” and “F” show the male and female mice, respectively. (g) Litter sizes of F1 × F1 intercrosses. Data are the mean ± standard deviation. N.S.: not significant.
