Figure 6 | Scientific Reports

Figure 6

From: Role of mitochondria in rescuing glycolytically inhibited subpopulation of triple negative but not hormone-responsive breast cancer cells

Figure 6The alternative text for this image may have been generated using AI.

Effects of IA on mitochondrial bioenergetics, transmembrane potential and ROS production. (A) The Seahorse mito-stress assay. OCR is measured before and after adding inhibitors/uncoupler to induce different mitochondrial respiratory states (defined in the shaded areas). (B) Basal respiration, proton leak, spare capacity, and non-mitochondrial respiratory rates (defined in A) were quantified and compared for both cell types after 4 or 12 h of treatment with 20 mM IA. (C,D) Effect of IA-treatment time on mitochondrial transmembrane potential as determined by flow cytometric analysis using TMRE staining for both MCF7 and MDA-MB-231 cells; respectively. (E,F) Flow cytometric analysis of cellular ROS levels using DCF cellular staining of both MCF7 and MDA-MB-231 cells; respectively.

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