Figure 6
From: New protein-DNA complexes in archaea: a small monomeric protein induces a sharp V-turn DNA structure
Binding of MC1 protein to two different DNA probes of 17 bp (*DNA15bp) and 25 bp (*DNA23bp). (a) Top: Electrophoresis gel of EMSA reaction mixtures (10 µl) prepared at 4 °C by mixing DNA (0.05 nM) and increasing MC1 protein concentrations from 0.04 nM to 6.6 nM. Bottom: Binding curves obtained from quantification using ImageQuant software of the EMSA experiments. The fraction of bound DNA is plotted as a function of protein concentration [MC1]. (b) Relaxation dispersion curves, fitted using the NESSY software, for Glu15 and Gly17 of the MC1 protein in complex with the DNA23bp. Data were fitted to a two-site, fast exchange model where pa and pb are the populations of the two state models (pa + pb = 1), kex is the exchange constant and δω is the chemical shift difference between states.
