Figure 3

ResA and ResB cells have a high tumour initiating and self-renewal potential. (a) Median in vivo tumour doubling rates, representative images, and engraftment rates of xenografts derived from LNCaP, ResA and ResB cells in male mice treated with 10 mg/kg enzalutamide or vehicle. The dashed line represents the median of vehicle treated LNCaP tumours. (b) Heat maps of high-resolution colony formation assays showing the formation of paraclones (low tumour initiating capacity), meroclones (intermediate) and holoclones (high tumour initiating capacity) in the cell lines in normal growth medium (containing 10 µM enzalutamide for ResA/ResB). (c) Heat map of the MSigDB Hallmark “Epithelial Mesenchymal Transition” gene signature expression and FPKM (fragments per kilobase million) mRNA expression of the mesenchymal marker VIM (Vimentin) in the cell lines in conditions similar to their respective growth medium (+10 nM DHT for all cell-lines; +10 µM enz for ResA/ResB). The error bars indicate the 95% confidence interval.