Table 3 Sequence of the primers used in the construction of rAF-IL12.

From: Evaluation of a Recombinant Newcastle Disease Virus Expressing Human IL12 against Human Breast Cancer

Primer

Sequence

Forward primer

5′GCGCGCTAGCTTACAGTTAGTTCACCTGTTTATCTAGTTAGAAAAAACACGGGTAGAAGAGTTCGGATCCCGATTGGCACATTCAAGGCGCAATACCACCATGTGTCACCAGCAGTTGGTCATCTC 3′

Reverse primer

5′AAGCTAGCCTATTAGGAAGCATTCAGATAGCTCATCACTCTATCAATAGTC 3′

F 1 Forward Primer

ACCAGTACACACCCCTTCCGAGTTA

F 1 Reverse Primer

ATCTGTCTTGCTAGCTTACAGTTAGTTCACCTGTTTATCT

F 2 Forward Primer

CAACACTATATAATGATCTGTCTTGCTAGCTTACAGTTAGTTCA

F 2 Reverse Primer

AATAAGCAATGCCCTGGACAAGTTATCGG

  1. *Abbreviation: F = Fragment.
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