Table 3 Optimized multiple reaction monitoring (MRM) method pairs and parameters for eicosanoids.
From: Circulating exosomes may identify biomarkers for cows at risk for metabolic dysfunction
Endogenous lipids | Q1 (Positive) | Q3 (Positive) | Q1 (Negative) | Q3 (Negative) |
|---|---|---|---|---|
PGE2 | 351.1 | 271.1 | ||
PGF2α | 353.2 | 309.0 | ||
PGD2 | 351.0 | 271.3 | ||
PGFM | 353.2 | 113.0 | ||
TXB2 | 369.0 | 169.1 | ||
PGF2α-EA | 380.4 | 62.0 | ||
Internal standards | Q1 (Positive) | Q3 (Positive) | Q1 (Negative) | Q3 (Negative) |
PGE2-d4 | 355.1 | 319.0 | ||
PGF2α-d4 | 357.2 | 313.0 | ||
PGD2-d4 | 355 | 193.2 | ||
PGFM | ||||
PGF2α-EA-d4 | 384.4 | 62.0 |
