Figure 1
RpS5b is a germline ribosomal protein and RpS5a is expressed mostly in somatic cells. (a) Protein sequence alignment comparing RpS5a and RpS5b to each other and to RpS5 orthologues in yeast and human. These proteins are highly homologous but diverge in their N-terminal regions. (b) Co-immunoprecipitation experiments. Immunoprecipitations were conducted with antisera recognizing RpS5a, RpS5b, and non-immune IgG (top captions), blotted, and probed with antibodies as indicated on the right. (c) Western blot showing that RpS5b is detected in the ovary (O) and testis (T) but not in female or male carcasses (f.carc and m.carc), while RpS5a is ubiquitously expressed. (d) Western blot showing that RpS5b is highly abundant relative to RpS5a in 0–2 h embryos (E2h), but both paralogs have relatively equal abundance in 0–16 h embryos (E16h). (e) Immunostaining experiments showing that RpS5b is primarily expressed in the germline cells, while RpS5a is primarily, but not entirely, expressed in follicle cells. RpS6 is equally abundant in both tissues. Scale bars, 20 μm.
