Figure 4

Influence of SCAP encapsulation in ECM hydrogels on their immunomodulatory properties. SCAP seeded on inserts, alone or encapsulated in S8, B8 or S/F hydrogels, were co-cultured with LPS-activated BV2 cells and the gene expression of tumor necrosis factor alpha (Tnf), inducible nitric oxide synthetase gene (Nos2) and arginase 1 gene (Arg1) was quantified by RT-qPCR. Nos2/Arg1 ratio was also calculated. Results were expressed relative to the LPS-activated BV2 cells (control) set at 100% (red dotted bar). Error bars represent the standard error of the mean (N = 4, n = 3). One-way ANOVA with Tukey post-hoc test, * p < 0.05.