Figure 6 | Scientific Reports

Figure 6

From: Predicting DNA damage foci and their experimental readout with 2D microscopy: a unified approach applied to photon and neutron exposures

Figure 6

2D γ-H2AX foci read-out. Predictions of the 2D readout of foci yields (from initial DSBs), after exposure to different doses of: photons (from the reference X-ray field) (a); secondary protons (b), 12C (c) and 16O (d) ions from the Hiroshima neutron spectrum. The readout is simulated for different combinations of the slice thickness Δz (0.5; 2 μm) and clustering radius r (0.5; 1 μm) in the 2D clustering algorithm. Errors are calculated as standard deviations among different simulation runs. Lines are a guide to the eye. As a reference, best fit curves to experimental data on foci yields scored at 30 minutes after exposure (Fig. 4) are shown in black for X-rays (a).

Back to article page