Figure 4 | Scientific Reports

Figure 4

From: CFH exerts anti-oxidant effects on retinal pigment epithelial cells independently from protecting against membrane attack complex

Figure 4

CFH protects RPE cells tight junctions from oxidative stress. (a) ARPE‐19 cultures were treated with 4-HNE (30 μM) in the presence or not of recCFH (300 nM) and the mitochondrial redox potential was analysed by the MTT colorimetric method 6 h after. Inos, Catalase (cat) and Gpx mRNA expression were investigated by reverse transcription quantitative polymerase chain reaction (RT-qPCR) experiments 6 h after 4-HNE (30 μM) or 4-HNE (30 μM) and recCFH (300 nM) ARPE-19 cells co-treatment. All data were presented as mean ± s.e.m. Statistical significance was assessed using Mann-Whitney test. *P < 0.05; **P < 0.01; NS = no significant. Scale bars: 50 μm. (b,c) ZO-1 immunostaining was altered in ARPE-19 (b) and iRPE (c) cells 6 h after 4-HNE (30 μM) treatment. Exposure to recCFH (300 nM) preserved ZO-1 immunostaining in both ARPE-19 and iRPE 4-HNE treated cells. Quantification of ZO-1 immunostaining length fragment showed longer immunostained fragments only with recCFH as compared to 4-HNE treatment for both ARPE-19 and iRPE cells. Scale bars: 50 μm.

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