Figure 2
From: Human mesenchymal stem cell sheets in xeno-free media for possible allogenic applications
Passaging influence. hUC-MSC sheet morphological observations using cell passages 4, 6, 8, 10 and 12 seeded at 3.2–4 × 103 cells/cm2. (a) Morphology of passage 4, 6, 8, 10 and 12 cells observed using phase-contrast microscopy before sheet detachment. (b) Successful fabrication of hUC-MSC sheets using passage 4, 6, 8 and 10 cells. In contrast, passage 12 cells detached as non-contiguous disconnected cellular structures. Scale bars = 100 μm.
