Figure 3
From: Human mesenchymal stem cell sheets in xeno-free media for possible allogenic applications
Cell seeding influence. Morphological observation, cell proliferation rate, and cell sheet fabrication for hUC-MSCs seeded at 5 × 104, 1 × 105 and 2 × 105 initial cell numbers. (a) Cells cultured on TRCD and observed prior to sheet detachment. (b) Cell numbers counted using a hemacytometer after cells were seeded on TRCD until hUC-MSC sheets are observed. (c) Cells detached as disconnected pieces at one day prior to confluence at 3, 4 and 5 days in 2 × 105, 1 × 105 and 5 × 104 initial cell seeded groups, respectively. Intact cell sheets were successfully fabricated at 4, 5 and 6 day for seeding densities of 2 × 105, 1 × 105 and 5 × 104 initial cell number groups, respectively. One day post-confluence, cultured cells spontaneously detach as aggregated forms without TRCD temperature changes at 5, 6 and 7 days for the 2 × 105, 1 × 105 and 5 × 104 initial cell seeded groups, respectively. Scale bars indicate 100 μm in (a). Scale bars indicate 1 cm in (c).
