Figure 5
From: Human mesenchymal stem cell sheets in xeno-free media for possible allogenic applications
Cell-cell structural analysis using immunohistochemistry (IHC) and transmission electron microscopy (TEM). Cells successfully detached as sheets from TRCD by temperature changes at 4 days after seeding. Cell sheets were stained with isotype negative control antibody (a), ECM ((b) fibronectin and (c) laminin), (d) cell junction β-catenin, and (e) stem cell surface marker (CD44) antibodies to confirm that cell sheets preserved their functional structures after detachment. In TEM images, hUC-MSC sheets preserved their (e) ECMs and (f) cell-cell junction structures after detachment. Red arrow = ECMs; yellow arrow = cell junctions in the hUC-MSC sheet. Scale bars indicate 100 μm in (a–d). Scale bars (d,e) indicate 5 μm and 1 μm, respectively.
