Figure 6
From: Human mesenchymal stem cell sheets in xeno-free media for possible allogenic applications
Cytokine analysis of human hepatocyte growth factor (HGF) and tumor necrosis factor-alpha (TNF-α) secreted from hUC-MSC sheets. hHGF (anti-inflammatory cytokine) and hTNF-α (pro-inflammatory cytokine) were detected in culture supernatant for cells cultured for 24 hours. (a) no significant differences in hHGF secretion in 2 × 105, 1 × 105, and 5 × 104 initial cell seeded groups. (b) hTNF-α barely detected and not significantly different in 2 × 105, 1 × 105 and 5 × 104 initial cell seeded groups. (c) Significant reductions in hHGF secreted from hUC-MSC sheets as passage increases. (d) hUC-MSC sheet fabricated using passage 4 cells secreted significantly lower amount of hTNF-α, compared to hUC-MSC sheet fabricated using passage 6, 8, 10, and 12 cells *p < 0.05.
