Figure 7
From: Human mesenchymal stem cell sheets in xeno-free media for possible allogenic applications
Implanted hUC-MSC sheet retention in vivo. hUC-MSC sheets implanted within subcutaneous tissue in immuno-deficient mice. (d,e) At 10 days after implantation, hUC-MSC transplanted subcutaneous tissue sites were harvested for histological observation. In H&E-stained images, (b) hu-MSC cell sheets were confirmed clearly in subcutaneous tissue implant sites compared to (a) normal subcutaneous tissue. Additionally, (c) abundant vascular structures are observed in cell sheet implanted groups. hUC-MSC sheets secrete human HGF on the transplanted areas at 1 day (f) and 10 days (g) post implantation. Black arrow = implanted cell sheet; blue arrow = blood vessels. Scale bars (a,b) and (c,f,g) indicate 100 μm, 50 μm, respectively. Scale bars (c,d) indicate 0.5 cm.
