Figure 1
From: Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength
Deletion of Panx1 in osteocytes increases bone mass. (A) Panx1 protein levels in calvaria bones from Panx1fl/fl and Panx1Δot mice at 4 months of age were assessed by Western blotting and β-actin was used as a loading control. (B) Representative images of whole body histological preparations (n = 3/genotype) stained by Alcian blue/alizarin red in 6-day-old mice for visual inspection of cartilage (blue) and calcified tissue (magenta). (C) The levels of the apoptosis-related genes were measured by qPCR bone marrow-flushed long bones obtained from female mice. Bars represent mean ± s.d., #p < 0.05 versus Panx1fl/fl mice at the same age, *p < 0.05 versus 4-month-old mice of the same genotype, and lines indicate age effect, by 2-way ANOVA. (D) Body weight and total body, spinal and femoral BMD were assessed monthly in female mice from 1 to 13 months of age by DXA (n = 9–12 per group). ¥p < 0.05 versus Panx1fl/fl mice at the same age by t-test.
