Figure 5
From: Age- and sex-dependent role of osteocytic pannexin1 on bone and muscle mass and strength
Panx1Δot female mice exhibited minimal changes in vertebral dynamic and static histomorphometric parameters. (A) MS/BS, MAR, and BFR/BS were measure in unstained sections of lumbar vertebra (n = 9–12 per group). (B) N.Oc/BS, Oc.S/BS, ES/BS were scored in vertebral cancellous bone after staining for TRAPase/Toluidine blue in Panx1fl/fl and Panx1Δot mice at 4 and 13 months of age (n = 9–12 per group). (C) N.Ob/BS and Ob.S/BS were scored in lumbar vertebra stained with von Kossa/McNeal (n = 9–12 per group). (D) Circulating markers of bone formation (P1NP, 9–12 per group and ALP, n = 9–12 per group) were measured in serum by ELISA. Bars represent mean ± s.d., #p < 0.05 versus Panx1fl/fl mice at the same age, and lines indicate age effect, by 2-way ANOVA.
