Figure 2

Experimental and numerical methodology for the analysis of cell cultures. First, cells of interest were selected using bright-field and/or fluorescence imaging. Then selected cells were mapped by means of high spectral resolution (<1 cm⁻¹) MCARS microspectroscopy in the 2500–3200 cm⁻¹ range (see Methods for details of the MCARS system). Finally, the vibrational information was extracted by using MEM algorithm, and cell images were reconstructed at 2850 cm⁻¹ (CH₂ symmetric stretching) and 2930 cm⁻¹ (CH₃ symmetric stretching).