Figure 1

Unaltered thymic Treg numbers but higher splenic Treg numbers in Nfkb2−/− mice. (A,B) Thymocytes isolated from WT (B6.SJL) and Nfkb2−/− mice were fixed, permeabilised and stained with anti-mouse CD45.1, CD45.2, CD90, CD4, CD8, CD25 and FOXP3 to identify Treg precursors (pre-Tregs) and mature Tregs (Supplementary Fig. S1). The bar graphs represent the (A) frequencies and (B) cell numbers per thymus of pre-Tregs and mature Tregs in CD90⁺CD4⁺CD8⁻ thymocytes (CD4SP: CD4 single-positive thymocytes). (C–G) Spleen cells isolated from WT and Nfkb2−/− mice were fixed, permeabilised and stained with anti-mouse CD45.1, CD45.2, CD4, CD25, FOXP3 and HELIOS to identify Tregs as well as the iTreg and nTreg subpopulations (Supplementary Fig. S2). The bar graphs represent (C) the frequencies and (D) numbers per spleen of the Tregs in total CD4 cells. The ratio of iTreg/nTreg within the total Tregs (E), and the frequencies (F) and numbers per spleen (G) of iTregs and nTregs in the spleens of WT and Nfkb2−/− mice are also shown. *p < 0.05; n ≥ 12 mice/group.