Figure 6

Nfkb2−/− central and effector Tregs show higher survival, activation and proliferation in vivo. (A–C) WT and Nfkb2−/− central Tregs were sorted from the spleens of bi-parental bone marrow chimeras and parked in vivo for 10 days. Transferred populations were then analysed (Supplementary Fig. S5). Bar graphs represent (A) frequencies of the indicated transferred Treg populations in the spleen of recipients at the end of 10 days, (B) frequencies of proliferating cells in respective transferred Treg population as indicated by Ki67 expression frequencies, and (C) ratios of central (CD62L^high) to effector (CD62L^low) Tregs. (D) WT and Nfkb2−/− central Tregs were sorted from the spleens of bi-parental bone marrow chimeras and parked in vivo for 3 days. The bar graph represents the frequencies of the indicated transferred Treg populations in the spleen of recipients at the end of 3 days. (E–G) WT and Nfkb2−/− effector Tregs were sorted from the spleens of bi-parental bone marrow chimeras and parked in vivo for 10 days as above. Bar graphs represent (E) frequencies of the indicated transferred Treg populations in the spleens of recipients at the end of 10 days, (F) frequencies of proliferating cells in respective transferred Treg populations as indicated by Ki67 expression frequencies, and (G) ratios of central (CD62L^high) to effector (CD62L^low) Tregs. *p < 0.05; n ≥ 4 mice/group.