Figure 6
From: High-affinity Cu(I) chelator PSP-2 as potential anti-angiogenic agent
Aortic ring assay. Qualitative assessment of outgrowth endothelial cells (A). DMSO treatment has no effect on network formation (left), while DMSO + 5 µM PSP-2 leads to fewer branches but longer sprouts in the chicken aortic ring assay (middle). With avastin, an anti-angiogenic agent, no sprouting was observed (right). Prevalent outgrowth structures are schematically shown below. Quantitative readouts (B), with number of sprouts within a region of interest (ROI); length of branches between two junctions and angle between branches, analyzed by Mann Whitney test. For comparison, sheep aortic ring assay was performed, showing similar effects of outgrowth patterns as found in the chicken aortic ring assay (see Supporting Information, SI Fig. 2).
