Figure 7
From: Bioassay for Endothelial Damage Mediators Retrieved by Hemoadsorption
Characterization of CytoSorb-derived protein fractions. Albumin contents (ng/ml, y-axis) in given fractions (x-axis) using a high-sensitive ELISA (Immulite1000®). Fractions F1–F18 and F26–F39 were below the detection limit (4 µg/ml). The highest amount of albumin was found in F20 (>6000 µg/ml). (a) Amounts of deoxyribonucleic acids (DNA) were determined by spectrophotometry at 260/280 nm (NanoDrop). The highest amounts of DNA were found in F13 (3915.6 ng/ml) (b) the insert shows the contents of oxidized DNA (8-hydroxy-2′-deoxyguanosine from DNA, 8-hydroxyguanosine from RNA and 8-hydroxyguanine from DNA or RNA); results were obtained by ELISA (Cayman Chemicals.com); the highest concentration was found in F11 (40.97 pg/ml). Advanced glycation end products (AGEs) were quantified by ELISA (mybiosource.com); the highest amount was found in F14 (0.95 µg/ml); means of duplicates’ analyses are shown. (c) Red-filled bars were fractions which consistently influenced endothelial viability, ATP contents, and apoptosis.
