Table 3 Summary of PERMANOVA results for the analysis of differences in endophyte community composition across the different factors (i.e., site, leaf status, Acer species and their interactions).

From: Deciphering bacterial and fungal endophyte communities in leaves of two maple trees with green islands

 

df*

Bacterial endophyte communities

Fungal endophyte communities

SS

MS

Pseudo-F

P -value

perm

SS

MS

Pseudo-F

P -value

perm

Source

Model 1 (entire dataset)

Acer species (Sp)

1

0.42

0.42

1.06

0.49

30

0.48

0.48

1.01

0.49

30

Leaf status (Le)

2

0.41

0.20

0.81

0.72

993

1.94

0.97

5.91

0.04 A

996

Site (Si)

2

1.45

0.72

2.55

0.001

998

1.28

0.64

3.38

0.001

999

SpxLe

2

0.57

0.28

1.16

0.47

999

1.31

0.65

2.20

0.26

998

SpxSi

1

0.47

0.47

1.67

0.056

997

0.69

0.69

3.65

0.005

998

LexSi

4

1.06

0.27

0.94

0.62

998

0.57

0.14

0.75

0.78

998

SpxLexSi

1

0.23

0.23

0.81

0.69

998

0.34

0.34

1.79

0.10

999

Residual

51

14.46

0.28

   

9.64

0.19

   

Total

64

20.20

    

20.09

    
 

Model 2 (entire dataset)

Acer species (Sp)

1

0.38

0.38

1.12

0.44

30

0.24

0.24

0.63

0.58

30

Green island presence (In)

1

0.29

0.29

0.77

0.66

333

0.54

0.54

2.16

0.21

341

Site (Si)

2

1.11

0.55

2.03

0.001

997

0.86

0.43

1.83

0.02

998

SpxIn

1

0.36

0.36

1.30

0.21

999

0.35

0.35

1.47

0.22

998

SpxSi

1

0.47

0.47

1.74

0.04

999

0.69

0.69

2.93

0.01

998

InxSi

2

0.75

0.37

1.37

0.10

998

0.41

0.21

0.87

0.58

998

Res

56

15.29

0.27

   

13.16

0.23

   

Total

64

20.20

    

20.09

    
 

Model 3 ( A. campestre )

Leaf status (Le)

2

0.51

0.25

0.93

0.57

972

1.97

0.99

6.83

0.02 A

965

Site (Si)

2

1.01

0.51

1.96

0.02

998

1.05

0.52

2.69

0.004

999

LexSi

3

0.82

0.27

1.06

0.37

997

0.42

0.14

0.72

0.75

999

Res

25

6.48

0.26

   

4.86

0.19

   

Total

32

9.22

    

9.41

    
 

Model 4 ( A. platanoides )

Leaf status (Le)

2

0.43

0.22

1.00

0.37

180

2.09

1.04

3.93

0.14

180

Site (Si)

1

1.26

1.26

4.09

0.002

998

1.74

1.74

9.47

0.001

999

LexSi

2

0.43

0.22

0.71

0.94

996

0.53

0.27

1.44

0.16

996

Res

26

7.99

0.31

   

4.78

0.18

   

Total

31

10.19

    

9.20

    
  1. Data were analysed with site as random effect and Acer species as well as leaf status (model 1) or presence of green islands (model 2) as fixed factors. Each Acer species was also tested separately with site as random and leaf status as fixed factor (models 3 and 4). Results of the PERMANOVA are based on Bray-Curtis dissimilarities with 999 permutations (perm).
  2. *The degree of freedom (df) is the same for bacteria and fungi.
  3. AMultiple comparison revealed a marginally significant difference between all GLA and YLA samples (t = 4.11, P = 0.09, permutations = 192) and a significant difference between GLA and YLA samples of A. campestre (t = 4.1, P = 0.048, permutations = 192).
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