Figure 1
Standard curve assessing the Limit of Detection (LOD) and Limit of Quantification (LOQ) for the qPCR assays detecting the DNA traces of I. nubecula. Both limits were calculated from a 1:10 serial dilution with 10 replicates per concentration. The LOD was 6.82 10−6 ng DNA µL−1 at 39.29 ± 2.00 Ct (i.e. Cycle threshold) and the LOQ was 6.82 10−4 ng DNA µL−1 at 34,48 ± 0,95 Ct (Slope = −3.86, Y inter = 19.52, R2 = 0.97, Eff% = 81.63).
