Figure 2

Map showing the 12 locations of the River Dee sampled with both kick-sampling and eDNA survey for monitoring I. nubecula in Wales, United Kingdom. Red dots are showing the sampled locations, half green circle the locations positive with eDNA detection using ddPCR, the half orange circle the locations were I. nubecula was found using kick-sampling. Locations W5 and W6 were not surveyed using kick-sampling method.