Table 1 Table depicting the kick-sampling results for I. nubecula (i.e. how many specimens found at each site), the eDNA results using ddPCR analysis (i.e. if one natural replicate was positive to I. nubecula DNA), the amount of time spent performing kick-sampling and eDNA sampling, the amount of water filtrated for all natural replicate at each site, the sampling date, pH, dissolved oxygen and GPS coordinate. The site inaccessible for conducting a kick-sampling were marked “ns”.
Sample ID | I. nubecula | eDNA (ddPCR) | Time (s) | Volume (ml) | Date | pH | O2 | Latitude | Longitude |
|---|---|---|---|---|---|---|---|---|---|
W1 | 0 | No | 60 | 350 | 31/03/2018 | 7.48 | 12.5 | 52.952759 | −3.0232733 |
W2 | 3 | Yes | 60 | 200 | 01/04/2018 | 7.53 | 11.9 | 53.024980 | −2.8760059 |
W3 | 30 | No | 60 | 700 | 09/03/2018 | 6.69 | 11.9 | 53.010679 | −2.8998019 |
W4 | 16 | Yes | 120 | 1000 | 09/03/2018 | 6.52 | 11.8 | 53.003120 | −2.9138314 |
W5 | ns | Yes | 45 | 750 | 15/03/2018 | 7.83 | 11.4 | 53.095257 | −2.8967275 |
W6 | ns | No | 60 | 300 | 01/04/2018 | 7.82 | 12.5 | 53.011702 | −2.8686273 |
W7 | 1 | Yes | 90 | 750 | 14/03/2018 | 7.67 | 11.6 | 52.978139 | −2.9627502 |
W8 | 1 | No | 90 | 750 | 14/03/2018 | 7.8 | 10.7 | 52.964635 | −2.9628967 |
W9 | 0 | No | 90 | 750 | 11/03/2018 | 6.75 | 11.8 | 52.945402 | −3.0194684 |
W10 | 0 | No | 60 | 300 | 31/03/2018 | 7.74 | 13 | 52.970460 | −3.0879607 |
W11 | 0 | No | 45 | 500 | 11/03/2018 | 6.63 | 11.6 | 53.100487 | −2.9239146 |
W12 | 0 | No | 90 | 750 | 15/03/2018 | 7.69 | 10.9 | 52.967603 | −3.0619060 |
