Table 2 Table depicting the eDNA detection results using qPCR and ddPCR techniques on diluted and undiluted (1:2) natural replicates (NR) sampled at each field location.

From: Combining ddPCR and environmental DNA to improve detection capabilities of a critically endangered freshwater invertebrate

Sample ID

qPCR

ddPCR

undiluted

undiluted

diluted

NR A

NR B

NR C

NR A

NR B

NR C

NR A

NR B

NR C

W1

—

—

—

—

—

—

—

—

—

W2

—

—

—

—

0.8

0.7

—

—

—

W3

—

—

—

—

—

—

—

—

—

W4

—

—

—

—

—

0.7

0.7

—

0.14

W5

—

—

—

0.7

0.7

0.14

—

—

0.6

W6

—

—

—

—

—

—

—

—

—

W7

—

—

—

—

—

—

—

0.7

—

W8

—

—

—

—

—

—

—

—

—

W9

—

—

—

—

—

—

—

—

—

W10

—

—

—

—

—

—

—

—

—

W11

—

—

—

—

—

—

—

—

—

W12

—

—

—

—

—

—

—

—

—

‘positive control’

—

5.4

8.2

  1. ‘—’ depict the absence of eDNA detection using qPCR and/or ddPCR. Quantification values of ddPCR results are displayed in copy per µL−1. Natural replicates were analysed using six technical replicates with qPCR and without replicates using ddPCR. All samples revealed a negative result for I. nubecula eDNA using qPCR. DNA from the targeted specie was amplified in samples from four field locations and in the ‘positive control’.
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