Figure 4 | Scientific Reports

Figure 4

From: Genome-wide association study and whole-genome sequencing identify a deletion in LRIT3 associated with canine congenital stationary night blindness

Figure 4

WT canine LRIT3 transcripts and expression of WT and mutant canine LRIT3 proteins. (a) RT-PCR showing the presence of full-length LRIT3 (large arrow head) as well as a shorter and significantly less abundant second isoform (small arrow head) in the WT canine retina. NTC, no template control. (b) Western blot showing expression of the expected full product (75 kD) with WT LRIT3 while the mutant LRIT3p.249* show expression of a truncated protein (29 kD) and lack of the full product. Images of the complete gel (a) and blot (b) are included in Supplementary Fig. 7.

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