Figure 3
PAX3-FOXO1 fusion oncoprotein alters the miRNA content of exosomes. (a) Hierarchical clustering of the deregulated miRNA P3F-C2C12 derived exosomes compared to Ctrl-C2C12 derived exosomes. Each column represents an exosome isolated replicate, and each row represents a miRNA. The scaled expression of each miRNA, denoted as the row Z-score, is plotted in green–red color scale. High expression levels are indicated in green and low expression levels are shown in red. (b) Histograms representing mean log fold change (logFC) of the indicated miRNA in P3F-C2C12 versus Ctrl-C2C12 derived exosomes. Results are shown using two different endogenous controls (Let-7a and snoRNA202), as indicated. Values are means of 3 independent exosome preparations, each run in duplicates. Bars represent standard deviation. (c) Histograms representing indicated miRNA levels in P3F-C2C12 cells versus Ctrl-C2C12 cells, normalized to snoRNA202 expression detected by qRT-PCR analysis. Values presented are means of three independent experiments. Bars represent standard deviation. Asterisks denote a statistically significant difference (p-value < 0.05). (d) IPA Network analysis of deregulated exosomal miRNA due to PAX3-FOXO1 transduction in C2C12 cells reveals 4 networks that contain more than one identified focus miRNA. These networks were generated through the use of IPA (QIAGEN Inc., https://www.qiagenbioinformatics.com/products/ingenuity-pathway-analysis).
