Figure 10
Relative abundance of differentially expressed proteins and functional clusters related with muscle contraction and microtubule based process during the differentiation developmental stage (day 8 vs day 12) in a gilthead sea bream primary muscle cell culture. On the top, the network analysis of protein-protein interactions among proteins grouped into two functional categories, according to Gene Ontology enrichment analysis by GOEAST. In these networks, nodes are proteins, the thickness of the line indicate the degree of confidence prediction of the interaction, according to the STRING databases66. On the bottom, the relative abundance of protein spots of each cluster are showed. Data are shown as mean ± SEM of % vol. Significant differences by t-test (p < 0.05). The average of local clustering coefficients were 1 for “Muscle contraction” (GO: 00069367, p = 3.85e-07) and for “Microtubule based process” (GO: 0007017). Protein acronyms correspond to Gene Symbol (see Supplementary Table S2 for details). The interactome of differentiation phase is represented in Fig. 6B, and more relevant data from the network stats are provided in Supplementary information (Table S4).
