Figure 3

2-DE master gels map showing the protein expression profiles from day 4 and day 8. Gels are loaded with 300 μg of total protein extract from the soluble muscle cell protein fractions. Proteins were separated in the first dimension on pH 3–10 non-linear IPG strips, followed by SDS-PAGE on 12.5% w/v gels. Gels were stained with colloidal CBB. Candidate spots listed on the gels were identified by MS. The images of full-length gels are shown in Supplementary information (Fig. S1: C2D4 of day 4 and C5D8 of day 8). Spots outlined in red and green indicate the proteins that were overexpressed and underexpressed respectively. Below each gel, the expression data for selected spots are shown in agglomerative hierarchical clusters of Z-score transformed intensity values (Perseus program). Cluster coloration indicates protein abundance in the sample (yellow: higher abundance, green: lower abundance, black: unchanged). C1, C2, C3, C4 and C5 represent five culture and D4 and D8 represent the day of culture: 4 or 8 days.