Figure 7
Functional clusters and abundance of differentially expressed proteins during the proliferation developmental stage (day 4 vs day 8) in a gilthead sea bream (Sparus aurata L.) primary muscle cell culture. On the top, the network analysis of protein-protein interactions among proteins grouped into 4 functional categories, according to Gene Ontology enrichment analysis by GOEAST. In this network, nodes are proteins, the thickness of the line indicate the degree of confidence prediction of the interaction, according to the STRING databases66. On the bottom, the relative abundance of protein spots of each cluster are showed. Data are shown as mean ± SEM of % vol. Significant differences by t-test (p < 0.05). The average of local clustering coefficients were: 0.521 for “Cytoeskeleton” (GO: 0005856, p = 0.00804), 0.667 for “Aminoacyl-tRNA ligase activity” (GO: 0004812, p = 1.02e-05), 0.833 for “proteosome-mediated ubiquitin-dependent protein catabolic process” (GO: 0043161, p = 0.000866), 0.805 for “Generation of precursor metabolites and energy” (GO: 0006091, p = 3.54e-07). Protein acronyms correspond to Gene Symbol (see Supplementary Table S1 for details).The interactome of proliferation phase is represented in Fig. 6A, and more relevant data from the network stats are provided in Supplementary information (Table S3).
