Table 1 Primers used for qPCR. Sequences, annealing temperature (Ta) and GenBank accession number.

From: Proteomic characterization of primary cultured myocytes in a fish model at different myogenesis stages

Gene

Primer sequence (5ā€²-3ā€²)

Ta (Ā°C)

Accession number

Reference

Myogenin

F: CAGAGGCTGCCCAAGGTCGAG

R: GAGGTGCTGCCCGAACTGGGCTCG

68

EF462191

19

EF1Ī±

F: CTTCAACGCTCAGGTCATCAT

R: GCACAGCGAAACGACCAAGGGGA

60

AF184170

19

RPL27a

F: AAGAGGAACACAACTCACTGCCCCAC

R: CTTGCCTTTGCCCAGAACTTTGTAG

68

AY188520

63

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