Figure 4
Virucidal activity of RES against extracellular ZIKV particles. Huh7 cells were infected with RES (80 µM)-pre-incubated ZIKV virus at an MOI of 1 for 48 h. The cell supernatants were collected after 48 h and virus titer was determined using focus-forming assay (A). Foci formed in mock- and pre-incubated RES treatment conditions after three days of incubation were shown (B). The mock-treated and non-infected cells were used as controls. The qRT-PCR assay was used to quantify the ZIKV mRNA copy numbers of mock-incubated and RES-pre-incubated virus conditions (C). Data obtained from duplicate assays were analyzed and plotted using Graph Pad Prism 7 (Graph Pad Software Inc., San Diego, CA, USA, 2016). Statistical differences between groups were as follow: *P < 0.05.
