Figure 6
RES antiviral effects on ZIKV protein. Protein was extracted from the ZIKV-infected cells treated with 80 µM of RES. Capture ELISA for detection of ZIKV protein was performed in 96-well plate. The plate was coated with the extracted protein overnight and then incubated with increasing dilution of 4g2 antibody, followed by detection of bound antigen-antibody with peroxidase-conjugated secondary antibody (goat anti-mouse IgG) and substrate. The graphs represented different modes of RES treatments (80 µM); prior to virus infection (pre-infection) (A) and after virus infection (post-infection) (B). The absorbance value was read at 450 nm.
