Table 1 Characteristics of 35 Taqman assays designed to assess presence/absence and DNA quantity of 40 plant species in 98 Savi’s pine vole stomach contents.

From: Assessment of seasonal variation of diet composition in rodents using DNA barcoding and Real-Time PCR

Genus and species	Forward primer (5′-3′)	GC %	bp	Tm	Reverse primer (5′-3′)	GC %	bp	Tm	PCR product (bp)	Taqman probe (5′-3′)	GC %	bp	Tm
Amaranthus retroflexus	AACATCGAGCCCGTTGCT	56	18	50	GGAAGTAAACATGTTAGTAACAGAACCTTCT	35	31	58	102	TTTGTTATGTAGCGTATCCTTT	32	22	47
Avena barbata	CGTCTGGAGGATCTACGAATTCC	52	23	57	CATGAGGCGGACCTTGGAAA	55	20	54	61	CCCTGCTTATACAAAAAC	39	18	43
Bellis perennis	CGATGGACTTACGAGCCTTGATC	52	23	57	CCAGGAACGGGCTCGATT	61	18	53	63	AAAGGGCGGTGCTATGG	59	17	49
Capsella bursa-pastoris	CCGTTCCAGGAGAAGAAACTCAA	48	23	55	ACATGTTAGTAACCGAACCTTCTTCAAA	36	28	56	84	TTTATTGCGTATGTAGCTT	32	19	42
Cardamine hirsuta	CACATCGAGCCCGTTCCA	61	18	53	ACATGTTAGTAACCGAACCTTCTTCAAA	36	28	56	94	AATTTATTGCATATGTAGCTT	24	21	43
Cirsium arvensis	TTACCAGCCTTGATCGGTACAAG	48	23	55	GGTCTAAAGGGTAAGCTACATAAGCAA	41	27	57	99	TCGAGCGCGTTATTGG	56	16	46
Cynodon dactylon	CTATCACATCGAACCCGTTCCT	50	22	55	ACAGAACCCTCTTCAAATAGATCTAATGGA	37	30	58	87	GGGAAGACAGTCAATATATCTG	41	22	51
Echinochloa crus-gallii	CGTTACAAAGGACGATGCTATCACA	44	25	56	GAACCCTCTTCAAATAGGTCTAATGGAT	39	28	57	101	GTTCCTGGGGAGCCAGAT	61	18	53
Elytrigia repens	CTATCACATCGAGCCTGTTCCT	50	22	55	ACGGAACCCTCTTCAAATAGGTCTA	44	25	56	87	CAATTTATCTGTTATGTAGCTT	27	22	46
Erigeron bonariensis	TCCAAGTTGAGAGAGATAAATTGAACAAGT	33	30	56	TTTAGCGGATAACCCCAATTTAGGTTT	37	27	55	86	CCCTGTTGGGCTGTACTA	56	18	50
Erigeron sumatrensis	TCCAAGTTGAGAGAGATAAATTGAACAAGT	33	30	56	TTTAGCGGATAACCCCAATTTAGGTTT	37	27	55	86	CCCTGTTGGGCTGTACTA	56	18	50
Geranium dissectum	CGTCTGGAGGATCTGCGAATC	57	21	56	TCAACTTGGATGCCGTGAGG	55	20	54	74	CTGCTTATGTGAAAACTT	33	18	41
Geranium pusillum	ATCTTCTACCGGTACATGGACAAC	46	24	56	GATGTGATAGCAGCGTCCTTTGTA	46	24	56	82	GGCTTACTAGTTTGGATCGT	45	20	50
Geranium rotundifolium	TCCTCAACCCGGAGTTCCA	58	19	53	ACCGGTAGAAGATTCAGCAGCTA	48	23	55	64	CTGAGGAAGCGGGTGCCGC	74	19	60
Hordeum bulbosum	CAGCCAATGGATCTGTTATGTAGCT	44	25	56	CCCAAATACGTTACCCACAATGGAA	44	25	56	97	TATTTGAGGAGGGTTCCGT	47	19	49
Lolium multiflorum	CTTACCAGTCTTGATCGTTACAAAGGA	41	27	57	AGCTACATAACAGATCCATTGGTTGTC	41	27	57	87	ATCATATCGAGCCTGTTG	44	18	46
Lolium perenne	CTTACCAGTCTTGATCGTTACAAAGGA	41	27	57	AGCTACATAACAGATCCATTGGTTGTC	41	27	57	87	ATCATATCGAGCCTGTTG	44	18	46
Malva neglecta	GGCGATGCTACCACATTGAG	55	20	54	ACAGAACCTTCTTCAAAAAGGTCTAAGG	39	28	57	94	CTGGAGAAGAAGAACAATATA	33	21	47
Matricaria chamomilla	GCTGCTATGGAATTGAGCCTGTT	48	23	55	GGTCTAATGGGTAAGCTACATAAGCAA	41	27	57	72	CCTGGAGAAGAGAATCA	47	17	45
Medicago lupulina	GACGCTGCTACCACATCGA	58	19	53	AGGTCTAAGGGATAAGCTACATAAGCA	41	27	57	76	TTGCTGGAGAAGAGAGTCA	47	19	49
Plantago lanceolata	GTCCGGCTCACGGGATC	71	17	54	CTAACAGAGGACGACCATACTTGT	46	24	56	63	CAAAGTGAGAGAGATAAATT	30	20	44
Plantago major	CTGTTATGTAGCTTACCCTTTAGACCTTT	38	29	57	GGGCTTTGAATCCAAATACATTTCCT	38	26	55	95	TTGAAGAAGGGTCTGTTACTAAC	39	23	52
Poa annua	GCTATCACATTGAGCCTGTTGCT	48	23	55	ACCCTCTTCAAATAGGTCTAATGGAT	38	26	55	83	GGGAAGATAACCAATGGA	44	18	46
Poa trivialis	CGTCTGGAGGATCTACGAATTCC	52	23	57	GTTCAACTTATCTCTTTCAACTTGGATACC	37	30	58	90	TGCTTATGCAAAAACTTTCCAA	32	22	47
Portulaca oleracea	CTTACCAGTCTTGATCGTTACAAAGGA	41	27	57	GGGTAAGCTACATAACAAATATATTGATTGTCT	30	33	57	92	ATCGATGCCGTTCCTG	56	16	46
Prunus dulcis	TTACTAACATGTTTACTTCCATTGTAGGT	31	29	54	CGCAAATCCTCCAGACGTAGAG	55	22	57	79	TTGGGTTCAAGGCCCTGCG	63	19	55
Prunus persica	TTACTAACATGTTTACTTCCATTGTAGGT	31	29	54	CGCAAATCCTCCAGACGTAGAG	55	22	57	79	TTGGGTTCAAGGCCCTGCG	63	19	55
Rumex crispus	GTGCTCTACGTTTGGAGGATTTG	48	23	55	CGGGCCTTGGAAAGTTTTCGTA	50	22	55	62	CGAATTCCTCCTGCTT	50	16	43
Rumex conglomeratus	TCTACGTTTGGAGGATTTGCGAAT	42	24	54	GAGGCGGGCCTTGGAA	69	16	51	62	CCTGCTTATACGAAAACT	39	18	43
Rumex obtusifolius	TCTACGTTTGGAGGATTTGCGAAT	42	24	54	GAGGCGGGCCTTGGAA	69	16	51	62	CCTGCTTATACGAAAACT	39	18	43
Senecio vulgaris	ACGGTATCCAAGTTGAAAGAGATAAATTGA	33	30	56	CGTAGTTTTTAGCGGATAGACCCAAT	42	26	56	99	ATGGTCGTCCTCTAATGGGAT	48	21	52
Setaria verticillata	CGTTACAAAGGACGATGCTATCACA	44	25	56	AACCCTCTTCAAATAGGTCTAATGGATA	36	28	56	100	GTTCCTGGGGAGGCAGA	65	17	52
Sonchus arvensis	CCCTGCGTGCTCTACGT	65	17	52	GCGGACCTTGGAAAGTTTTAACATA	40	25	54	69	GAAGATTTACGAATCCCTA	37	19	45
Sonchus asper	CCCTGCGTGCTCTACGT	65	17	52	GCGGACCTTGGAAAGTTTTAACATA	40	25	54	69	GAAGATTTACGAATCCCTA	37	19	45
Stachys arvensis	TCGTTACAAAGGGCGATGCT	50	20	52	AGGTCTAAAGGGTAAGCTACATAACAG	41	27	57	87	CACATCGAGACCGTTCTT	50	18	48
Taraxacum officinale	CGTGCTCTACGTCTGGAAGATTTG	50	24	57	GCGGACCTTGGAAAGTTTTAACAT	42	24	54	64	CGAATCCCTGTTGCGT	56	16	46
Trifolium pratense	CCTTAGACCTTTTTGAAGAAGGTTCTGT	39	28	57	CGTAGAGCACGCAAGGC	65	17	52	91	CATGTTTACCTCTATTGTAGG	38	21	49
Trifolium repens	TGTAGGTAATGTATTTGGGTTCAAGGC	41	27	57	GAGGAGGACCTTGGAAAGTTTTAACA	42	26	56	98	CTACGCCTGGAAGATTT	47	19	45
Veronica agrestis	GGCGCTGCGGTAGCA	73	15	50	CCATCGGTCCACACAGTTGTC	57	21	56		ATGTACCAGTCGAAGATC	44	18	46
Veronica persica	GGCGCTGCGGTAGCA	73	15	50	CCATCGGTCCACACAGTTGT	55	20	54	59	ATCTTCAACCGGTACATGG	47	19	49

Plant species for which only a genus-specific Taqman assay was designed are reported in bold.

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Table 1 Characteristics of 35 Taqman assays designed to assess presence/absence and DNA quantity of 40 plant species in 98 Savi’s pine vole stomach contents.

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