Figure 1 | Scientific Reports

Figure 1

From: Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle

Figure 1

Nesprin-1-alpha2 pulls down kinesin light-chain from human myotube extracts, only if the STAR domain (with LEWD) is present. No pull-down of emerin or lamin A/C was detected. Extracts of human myotubes (Input) and the component of the myotube extract pulled-down by wild type N1α2 (Wt) or STAR deletion N1α2 (Star del) were probed with (a) KLC1 (rabbit pAb, GeneTex, GTX114510), (b) KLC1 (mouse mAb, Santa Cruz, sc-515792), (c) MANNEM1, emerin mAb, (d) MANNLAC1, Lamin A/C mAb or (e) mAb against GST tag as loading control. Whole blots are shown; black vertical lines separate the blue markers (taken with visible light) from the western blot (by chemiluminescence). In some blots (b–d), the markers were visible in the chemiluminescent exposure, so a separate visible-light exposure was not needed. Black arrows indicate bands of expected size.

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