Figure 2 | Scientific Reports

Figure 2

From: Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle

Figure 2

Nesprin-1-alpha2 pulls down kinesin light-chain from mouse C2C12 myotube extracts, only if the STAR domain (with LEWD) is present. Extracts of mouse C2C12myotubes (Input) and the component of the myotube extract pulled-down by wild type N1α2 (Wt) or STAR deletion N1α2 (Star del) were probed with (a) KLC (63–90) mAb or (b) mAb against GST tag as loading control. Whole blots are shown; black vertical lines separate the blue markers (taken with visible light) from the western blot (by chemiluminescence). In blot (a), the markers were visible in the chemiluminescent exposure, so a separate visible-light exposure was not needed. Black arrows indicate bands of expected size.

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