Figure 6 | Scientific Reports

Figure 6

From: Nesprin-1-alpha2 associates with kinesin at myotube outer nuclear membranes, but is restricted to neuromuscular junction nuclei in adult muscle

Figure 6

Nesprin-1-alpha2 is mainly restricted to neuromuscular junction nuclei in adult rat skeletal muscle and to cardiomyocyte nuclei in adult rat heart. Neuromuscular junctions were identified in transverse sections of rat intercostal muscle by labelling nicotinic acetylcholine receptors with alpha-bungarotoxin-TRITC (b,e,h). Nesprin-1-alpha2 (N1α2: a,c) was found almost-exclusively on nuclei associated with neuromuscular junctions, whereas total nesprin-1 (mAb MANNES1A) (d,f) was more widespread. Kinesin light-chain (g,h,i) was also found on many nuclei. Cardiomyocytes in sections of rat heart were identified by staining the sarcolemma for dystrophin (k,n). Examples of cardiomyocyte nuclei (large vertical arrows) and non-cardiomyocytes (small horizontal arrows). Nesprin-1-alpha2 (j,l) was predominantly on the nuclei of cardiomyocytes whereas total nesprin-1 (m,o) was also on nuclei of interstitial cells between cardiomyocytes.

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