Table 1 sgRNA sequence and the primers used to generate the templates for gRNA and Cas9 in vitro transcription.

From: Identification of the Sex of Pre-implantation Mouse Embryos Using a Marked Y Chromosome and CRISPR/Cas9

Primer

Sequence(5′-3′)

sgRNA

AGACTAGAGAGGCTCAATTCTGG

sgRNA-IVT-F

TAATACGACTCACTATAGGGAGACTAGAGAGGCTCAATTCG

TTTTAGAGCTAGAAATAG

sgRNA-IVT-R

AAAAGCACCGACTCGGTGCC

CAS9 IVT F

TAATACGACTCACTATAGGGAGATTTCAGGTTGGACCGGTG

CAS9 IVT R

GACGTCAGCGTTCGAATTGC

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